Analysis of total and transferrin-bound iron in human serum for pharmacokinetic studies of iron-sucrose formulations.
Bioanalysis
; 3(16): 1837-46, 2011 Aug.
Article
em En
| MEDLINE
| ID: mdl-21877893
ABSTRACT
BACKGROUND:
Patients with iron-deficiency anemia benefit from intravenous iron therapies. Development of these pharmaceutical agents requires pharmacokinetic studies monitoring levels of both the administered agent and transferrin-bound iron (TBI). Successful pharmacokinetic methods must discriminate iron species.RESULTS:
Routine colorimetric procedures were used to reliably measure total iron and TBI following iron-sucrose administration. Iron was liberated from iron-sucrose allowing the determination of all circulating iron. Solid-phase sample processing allowed the measurement of TBI. Circulating iron-sucrose could then be calculated as the difference between total iron and TBI.CONCLUSION:
A reproducible and robust spectrophotometric method was developed and validated for measuring total iron and TBI in human serum following iron-sucrose therapy.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Transferrina
/
Compostos Férricos
/
Anemia Ferropriva
/
Ferro
Limite:
Female
/
Humans
Idioma:
En
Ano de publicação:
2011
Tipo de documento:
Article