Construction of a neo fusion gene for expression in both prokaryotic and eukaryotic cells.
Gene
; 88(2): 279-83, 1990 Apr 16.
Article
em En
| MEDLINE
| ID: mdl-2189789
ABSTRACT
A high-copy-number plasmid, pLink, was constructed to allow the direct selection in Escherichia coli of a neo fusion gene capable of conferring Geneticin (G418) resistance on mouse L cells. pLink was derived from pdMmtneo by insertion of a KpnI linker within the 5'-coding region of the neo gene. This created a minus-one frameshift mutation resulting in a translational termination within the N-terminal region of the protein. The Neo activity was restored by insertion into the modified neo gene of a piece of coding sequence derived from human HPRT cDNA. The resulting plasmid, pAH, was microinjected into mouse A9 cells and shown to confer resistance to G418.
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Base de dados:
MEDLINE
Assunto principal:
Plasmídeos
/
Proteínas Recombinantes de Fusão
/
Resistência Microbiana a Medicamentos
/
Hipoxantina Fosforribosiltransferase
Limite:
Animals
/
Humans
Idioma:
En
Ano de publicação:
1990
Tipo de documento:
Article