Tumour-related expression of a translation-elongation factor-like protein.
Oncogene
; 5(6): 839-43, 1990 Jun.
Article
em En
| MEDLINE
| ID: mdl-2193292
ABSTRACT
We have identified a tumour-related 43 kd cytoplasmic protein (LC/p43) using a monoclonal antibody against the total proteins of human hepatoma cell line PLC/PRF/5. LC/p43 is preferentially expressed in a variety of tumours of human and animal origin, whereas no expression was detected in several normal adult tissues tested. LC/p43 expression was induced in rodent fibroblasts upon transfection with several viral oncogenes. Expression in non-transformed peripheral blood lymphocytes could be induced by treatment with phytohaemagglutinin (PHA) and subsequent culture with interleukin II, whereas retinoic acid treatment of transformed cells caused a drastic reduction of the antigen in the cells. Sequence analysis of three tryptic peptides of LC/p43 revealed 50-70% homology to different domains of the eukaryotic and prokaryotic translation-elongation factors EF1-alpha and EF-Tu, respectively.
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Base de dados:
MEDLINE
Assunto principal:
Fatores de Alongamento de Peptídeos
Tipo de estudo:
Prognostic_studies
Limite:
Animals
/
Humans
Idioma:
En
Ano de publicação:
1990
Tipo de documento:
Article