Toll-like receptor-3 ligation-induced indoleamine 2, 3-dioxygenase expression in human trophoblasts.
Endocrinology
; 152(12): 4984-92, 2011 Dec.
Article
em En
| MEDLINE
| ID: mdl-21952237
ABSTRACT
Indoleamine 2,3-dioxygenase (IDO) is an enzyme that degrades an essential amino acid, tryptophan, and plays a role in inhibiting the proliferation of T cells and intracellular pathogens. Inhibiting IDO in mice leads to fetal rejection, suggesting its significance in establishing pregnancy. Toll-like receptor 3 (TLR-3) is a key component of the innate immune system that recognizes viral double-stranded RNA and triggers immune reactions by producing type I interferon. Using a human trophoblast cell culture system, we studied the effect of TLR-3 ligation on IDO expression and function by treating trophoblasts with polyinosinic-polycytidylic acid [poly(IC)] (a synthetic double stranded RNA, which mimics viral RNA). Real-time PCR and Western blot analysis revealed that IDO mRNA and protein expression was significantly induced by poly(IC). The activity of IDO was also increased by poly(IC) given that the L-kynurenine concentrations were elevated in conditioned media. Conditioned media from poly(IC)-treated trophoblasts were found to inhibit the proliferation of human T cells significantly. Poly(IC) was also shown to induce interferon (IFN)-ß mRNA expression in trophoblasts. Recombinant human IFN-ß increased IDO mRNA expression in trophoblasts more rapidly than poly(IC). Pretreating with neutralizing antibody against IFN-ß significantly suppressed IDO induction by poly(IC). Collectively we have demonstrated that ligation of TLR-3 by poly(IC) induces IDO expression in human first-trimester trophoblasts via an IFN-ß-dependent pathway. These findings suggest that upon viral infection, trophoblasts induce IDO and in turn contribute to antimicrobial activity and maintenance of fetomaternal tolerance.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Trofoblastos
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Interferon beta
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Indolamina-Pirrol 2,3,-Dioxigenase
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Receptor 3 Toll-Like
Limite:
Female
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Humans
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Pregnancy
Idioma:
En
Ano de publicação:
2011
Tipo de documento:
Article