Identification and Role of Regulatory Non-Coding RNAs in Listeria monocytogenes.
Int J Mol Sci
; 12(8): 5070-9, 2011.
Article
em En
| MEDLINE
| ID: mdl-21954346
ABSTRACT
Bacterial regulatory non-coding RNAs control numerous mRNA targets that direct a plethora of biological processes, such as the adaption to environmental changes, growth and virulence. Recently developed high-throughput techniques, such as genomic tiling arrays and RNA-Seq have allowed investigating prokaryotic cis- and trans-acting regulatory RNAs, including sRNAs, asRNAs, untranslated regions (UTR) and riboswitches. As a result, we obtained a more comprehensive view on the complexity and plasticity of the prokaryotic genome biology. Listeria monocytogenes was utilized as a model system for intracellular pathogenic bacteria in several studies, which revealed the presence of about 180 regulatory RNAs in the listerial genome. A regulatory role of non-coding RNAs in survival, virulence and adaptation mechanisms of L. monocytogenes was confirmed in subsequent experiments, thus, providing insight into a multifaceted modulatory function of RNA/mRNA interference. In this review, we discuss the identification of regulatory RNAs by high-throughput techniques and in their functional role in L. monocytogenes.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
RNA Bacteriano
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Regulação Bacteriana da Expressão Gênica
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RNA não Traduzido
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Listeria monocytogenes
Tipo de estudo:
Diagnostic_studies
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Prognostic_studies
Idioma:
En
Ano de publicação:
2011
Tipo de documento:
Article