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A biochemical fluorometric method for assessing the oxidative properties of HDL.
Kelesidis, Theodoros; Currier, Judith S; Huynh, Diana; Meriwether, David; Charles-Schoeman, Christina; Reddy, Srinivasa T; Fogelman, Alan M; Navab, Mohamad; Yang, Otto O.
Afiliação
  • Kelesidis T; Department of Medicine, University of California, Los Angeles, CA 90095. Electronic address: tkelesidis@mednet.ucla.edu.
  • Currier JS; Department of Medicine, University of California, Los Angeles, CA 90095.
  • Huynh D; Department of Medicine, University of California, Los Angeles, CA 90095.
  • Meriwether D; Department of Obstetrics and Gynecology, University of California, Los Angeles, CA 90095.
  • Charles-Schoeman C; Department of Medicine, University of California, Los Angeles, CA 90095.
  • Reddy ST; Department of Medicine, University of California, Los Angeles, CA 90095; Department of Obstetrics and Gynecology, University of California, Los Angeles, CA 90095; Department of Molecular and Medical Pharmacology, University of California, Los Angeles, CA 90095 and.
  • Fogelman AM; Department of Medicine, University of California, Los Angeles, CA 90095.
  • Navab M; Department of Medicine, University of California, Los Angeles, CA 90095.
  • Yang OO; Department of Medicine, University of California, Los Angeles, CA 90095; Department of Microbiology, Immunology, and Molecular Genetics, David Geffen School of Medicine, University of California, Los Angeles, CA 90095.
J Lipid Res ; 52(12): 2341-2351, 2011 Dec.
Article em En | MEDLINE | ID: mdl-21957198
ABSTRACT
Most current assays of HDL functional properties are cell-based. We have developed a fluorometric biochemical assay based on the oxidation of dihydrorhodamine 123 (DHR) by HDL. This cell-free assay assesses the intrinsic ability of HDL to be oxidized by measuring increasing fluorescence due to DHR oxidation over time. The assay distinguishes the oxidative potential of HDL taken from different persons, and the results are reproducible. Direct comparison of this measurement correlated well with results obtained using a validated cell-based assay (r(2) = 0.62, P < 0.001). The assay can be scaled from a 96-well format to a 384-well format and, therefore, is suitable for high-throughput implementation. This new fluorometric method offers an inexpensive, accurate, and rapid means for determining the oxidative properties of HDL that is applicable to large-scale clinical studies.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Fluorometria / Lipoproteínas HDL Limite: Animals / Female / Humans / Male Idioma: En Ano de publicação: 2011 Tipo de documento: Article
Texto completo
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XML
PubMed Links
Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Fluorometria / Lipoproteínas HDL Limite: Animals / Female / Humans / Male Idioma: En Ano de publicação: 2011 Tipo de documento: Article