Effect of protein C gene mutation on coagulation and inflammation in hemorrhagic shock.
J Surg Res
; 175(1): 18-23, 2012 Jun 01.
Article
em En
| MEDLINE
| ID: mdl-21962741
ABSTRACT
INTRODUCTION:
Trauma patients are at high risk of complications and death from coagulopathy and inflammatory organ failure. Recent evidence implicates protein C (PC) as a key mediator of this process. We hypothesized that a mutation in the PC gene would ameliorate the inflammatory and coagulopathic response to hemorrhagic shock (HS) and resuscitation.METHODS:
FHH wild type and PC mutant rats underwent controlled hemorrhage for 120 min with 70% of blood volume removed. Rats were resuscitated with Ringers lactate (2x shed blood volume) and shed blood. Animals were sacrificed 4 h post-HS. Controls were untreated naïve rats.RESULTS:
AST and NFkB lung protein levels were elevated similarly in both WT and mutants compared with naïve rats. Plasma fibrinogen levels decreased significantly with progression of HS compared with baseline (BL) levels and returned towards normal 4 h after resuscitation. PC activity was similar in both groups at BL (0.5 ± 0.08 versus 0.6 ± 0.14; P = 0.14) and decreased from BL by 53% ± 24% in WT (P =0.08), by 67% ± 11% in mutants (P = 0.03) at sacrifice, and was not different between groups (P = 0.29).CONCLUSIONS:
Our model of HS and resuscitation produced a hypocoaguable, hyperinflammatory state with increased levels of NFkB and decreased levels of fibrinogen and PC levels. The mutated PC did not appear to alter these responses in our model of HS and resuscitation.
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Choque Hemorrágico
/
Coagulação Sanguínea
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Proteína C
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Inflamação
Limite:
Animals
Idioma:
En
Ano de publicação:
2012
Tipo de documento:
Article