Surrogate reporters for enrichment of cells with nuclease-induced mutations.
Nat Methods
; 8(11): 941-3, 2011 Oct 09.
Article
em En
| MEDLINE
| ID: mdl-21983922
ABSTRACT
Zinc-finger nucleases (ZFNs) and TAL-effector nucleases (TALENs) are powerful tools for creating genetic modifications in eukaryotic cells and organisms. But wild-type and mutant cells that contain genetic modifications induced by these programmable nucleases are often phenotypically indistinguishable, hampering isolation of mutant cells. Here we show that transiently transfected episomal reporters encoding fluorescent proteins can be used as surrogate genes for the efficient enrichment of endogenous gene-modified cells by flow cytometry.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Desoxirribonucleases
/
Mutação
Limite:
Humans
Idioma:
En
Ano de publicação:
2011
Tipo de documento:
Article