A triple spin-labeling strategy coupled with DEER analysis to detect DNA modifications and enzymatic repair.
Chembiochem
; 12(17): 2560-3, 2011 Nov 25.
Article
em En
| MEDLINE
| ID: mdl-21984076
ABSTRACT
In a spin Spin-labeled oligonucleotides produced by click chemistry can be studied by EPR, by using a DEER sequence. This was used to test a complex triple-labeling strategy with damaged DNA. Extensive and accurate analysis of DNA structure and enzymatic repair processes were performed after digestion by EndoIV. Modified DNA structures and DNA-protein interactions can now be readily studied.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Marcadores de Spin
/
DNA
/
Desoxirribonuclease IV (Fago T4-Induzido)
Idioma:
En
Ano de publicação:
2011
Tipo de documento:
Article