Chromatographic enzyme immunoassay for T-2 toxin.
J Immunol Methods
; 131(1): 77-82, 1990 Jul 20.
Article
em En
| MEDLINE
| ID: mdl-2199580
ABSTRACT
Both the active ester and maleimide moieties of the cross-linking reagent, N-[(gamma-maleimidobutyryl)oxy]succinimide (GMBS), were found to react with the primary amino groups on ribonuclease (RNase). This largely inactivated RNase towards a polymeric (but not monomeric) substrate. Citraconylating the RNase first, so that essentially only a single primary amino group remained to react with GMBS, overcame this problem. The subsequent maleimido-citraconyl-RNase was used to prepare a 11.1 M conjugate of anti-T-2 toxin Fab' and RNase (Fab'-RNase) in a 76% yield. The conjugate was used to detect as little as 0.1 microgram of T-2 toxin based on the ability of T-2 toxin to specifically displace Fab'-RNase complexed to a T-2 agarose affinity gel.
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Base de dados:
MEDLINE
Assunto principal:
Sesquiterpenos
/
Toxina T-2
/
Técnicas Imunoenzimáticas
Idioma:
En
Ano de publicação:
1990
Tipo de documento:
Article