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Enrichment of breast cancer stem cells using a keratinocyte serum-free medium.
Liu, Zhen-Zhen; Chen, Ping; Lu, Zhen-Duo; Cui, Shu-de; Dong, Zi-Ming.
Afiliação
  • Liu ZZ; Department of Pathophysiology, Basic Medical College, Zhengzhou University, Zhengzhou, Henan 450053, China.
Chin Med J (Engl) ; 124(18): 2934-6, 2011 Sep.
Article em En | MEDLINE | ID: mdl-22040505
BACKGROUND: Keratinocyte serum-free medium (K-SFM) is a defined medium used to support the growth of primary keratinocytes and embryonic stem cell. The aim of this research was to optimize enrichment of breast cancer stem cells (CSCs) using K-SFM. METHODS: A K-SFM was used to enrich CSCs from two breast cancer cell lines and a primary culture of breast cancer. RPMI-1640 supplemented with 10% fetal calf serum (FCS) was used as a control. CSCs were identified with flow cytometry using CD44(+)/CD24(-) as molecular markers. The expression of a variety of CSC markers (Oct-4, ABCG2, Nanog, N-cadherin, and E-cadherin) was analyzed with real-time PCR. RESULTS: Much higher percentage of CSCs was achieved with K-SFM: 17.3% for MCF-7 cells, 17.4% for SKBR-3, and 20.0% for primary breast cancer culture. Less than 1% CSC was achieved using RPMI-1640 supplemented with 10% FCS. In comparison to the CSCs obtained with RPMI-1640, CSCs in the K-SFM expressed higher levels of Oct-4, ABCG2, Nanog and N-cadherin, and lower level of E-cadherin. CONCLUSION: K-SFM is an optimal culture medium to maintain and to enrich breast CSCs.
Assuntos
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Base de dados: MEDLINE Assunto principal: Células-Tronco Neoplásicas / Queratinócitos / Meios de Cultura Livres de Soro / Técnicas de Cultura de Células Tipo de estudo: Prognostic_studies Limite: Female / Humans Idioma: En Ano de publicação: 2011 Tipo de documento: Article
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Base de dados: MEDLINE Assunto principal: Células-Tronco Neoplásicas / Queratinócitos / Meios de Cultura Livres de Soro / Técnicas de Cultura de Células Tipo de estudo: Prognostic_studies Limite: Female / Humans Idioma: En Ano de publicação: 2011 Tipo de documento: Article