Hepatic steatosis prevents heme oxygenase-1 induction by isoflurane in the rat liver.
World J Gastroenterol
; 17(37): 4184-90, 2011 Oct 07.
Article
em En
| MEDLINE
| ID: mdl-22072849
ABSTRACT
AIM:
To characterize the inductive effects of isoflurane (ISO) on hepatic heme oxygenase-1 (HO-1) in an animal model of hepatic steatosis.METHODS:
Lean (LEAN) and obese (FAT) Zucker rats were randomized into 4 groups 1 LEAN + pentobarbital sodium (PEN); 2 LEAN + ISO; 3 FAT + PEN; 4 FAT + ISO. The animals were mechanically ventilated for 6 h. In vitro analyses of liver tissue included determination of HO-1 mRNA and protein expression as well as measurement of HO enzyme activity and immunohistochemical analyses.RESULTS:
Compared to PEN treatment, ISO administration profoundly induced hepatic HO-1 mRNA and protein expression and significantly increased HO enzyme activity in lean Zucker rats. In contrast, no difference in HO-1 gene expression was observed after ISO or PEN anesthesia in obese Zucker rats.CONCLUSION:
The present study demonstrates that ISO is an inducer of hepatic HO-1 gene expression in non-steatotic organs but failed to upregulate HO-1 in steatotic livers.Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Indução Enzimática
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Anestésicos Inalatórios
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Heme Oxigenase-1
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Fígado Gorduroso
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Isoflurano
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Fígado
Limite:
Animals
Idioma:
En
Ano de publicação:
2011
Tipo de documento:
Article