Cyanine fluorophore derivatives with enhanced photostability.
Nat Methods
; 9(1): 68-71, 2011 Nov 13.
Article
em En
| MEDLINE
| ID: mdl-22081126
ABSTRACT
Fluorescence applications requiring high photostability often depend on the use of solution additives to enhance fluorophore performance. Here we demonstrate that the direct or proximal conjugation of cyclooctatetraene (COT), 4-nitrobenzyl alcohol (NBA) or Trolox to the cyanine fluorophore Cy5 dramatically enhanced fluorophore photostability without otherwise affecting its native spectral characteristics. Such conjugation is a powerful means of improving the robustness of fluorescence-based applications demanding long-lived, nonblinking fluorescence emission.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Carbocianinas
/
Corantes Fluorescentes
Limite:
Animals
/
Humans
Idioma:
En
Ano de publicação:
2011
Tipo de documento:
Article