Improved methods for genotype determination of human alcohol dehydrogenase (ADH) at ADH 2 and ADH 3 loci by using polymerase chain reaction-directed mutagenesis.
Clin Chem
; 36(10): 1765-8, 1990 Oct.
Article
em En
| MEDLINE
| ID: mdl-2208651
ABSTRACT
The human gene for producing alcohol dehydrogenase (ADH; EC 1.1.1.1) is polymorphic at ADH 2 and ADH 3 loci. Until now, the study of this polymorphism required liver biopsy or allele-specific radioactive probes. We have used directed mutagenesis by the polymerase chain reaction (PCR) to amplify and analyze the genotype of ADH 2 and ADH 3 loci. Thus, we could determine easily and unambiguously the complete genotype at these two loci by using a microsample of blood and restriction fragment length polymorphism after DNA amplification by PCR.
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Base de dados:
MEDLINE
Assunto principal:
Álcool Desidrogenase
/
Alelos
/
Isoenzimas
Limite:
Humans
Idioma:
En
Ano de publicação:
1990
Tipo de documento:
Article