COLT-Cancer: functional genetic screening resource for essential genes in human cancer cell lines.
Nucleic Acids Res
; 40(Database issue): D957-63, 2012 Jan.
Article
em En
| MEDLINE
| ID: mdl-22102578
ABSTRACT
Genome-wide pooled shRNA screens enable global identification of the genes essential for cancer cell survival and proliferation and provide a 'functional genetic' map of human cancer to complement genomic studies. Using a lentiviral shRNA library targeting approximately 16,000 human genes and a newly developed scoring approach, we identified essential gene profiles in more than 70 breast, pancreatic and ovarian cancer cell lines. We developed a web-accessible database system for capturing information from each step in our standardized screening pipeline and a gene-centric search tool for exploring shRNA activities within a given cell line or across multiple cell lines. The database consists of a laboratory information and management system for tracking each step of a pooled shRNA screen as well as a web interface for querying and visualization of shRNA and gene-level performance across multiple cancer cell lines. COLT-Cancer Version 1.0 is currently accessible at http//colt.ccbr.utoronto.ca/cancer.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Genes Essenciais
/
Bases de Dados Genéticas
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Interferência de RNA
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Genes Neoplásicos
/
Neoplasias
Tipo de estudo:
Diagnostic_studies
/
Prognostic_studies
/
Screening_studies
Limite:
Humans
Idioma:
En
Ano de publicação:
2012
Tipo de documento:
Article