Characterization of membrane proteins in isolated native cellular membranes by dynamic nuclear polarization solid-state NMR spectroscopy without purification and reconstitution.
Angew Chem Int Ed Engl
; 51(2): 432-5, 2012 Jan 09.
Article
em En
| MEDLINE
| ID: mdl-22113890
ABSTRACT
Membrane proteins in their native cellular membranes are accessible by dynamic nuclear polarization magic angle spinning solid-state NMR spectroscopy without the need of purification and reconstitution (see picture). Dynamic nuclear polarization is essential to achieve the required gain in sensitivity to observe the membrane protein of interest.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Ressonância Magnética Nuclear Biomolecular
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Proteínas de Escherichia coli
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Escherichia coli
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Proteínas de Membrana
Idioma:
En
Ano de publicação:
2012
Tipo de documento:
Article