Production of p-acetaminophenol by whole-cell catalysis using Escherichia coli overexpressing bacterial aryl acylamidase.
Biotechnol Lett
; 34(4): 677-82, 2012 Apr.
Article
em En
| MEDLINE
| ID: mdl-22130742
ABSTRACT
Aryl acylamidase (EC 3.5.1.13, AAA) acts on the amide bond between aryl and acyl groups. Whole cells of Escherichia coli overexpressing a novel bacterial AAA synthesized p-acetaminophenol (p-AAP) from p-aminophenol (p-AP, aryl compound) and acetate (acyl donor). Optimum conditions were pH 5.5 and 35°C with 100 mM p-AP and 600 mM sodium acetate in 100 mM sodium phosphate buffer including 1% (v/v) Triton X-100 for 60 h. 13.1 g p-AAP l(-1) was produced with a conversion yield of 87%.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Expressão Gênica
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Escherichia coli
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Amidoidrolases
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Acetaminofen
Idioma:
En
Ano de publicação:
2012
Tipo de documento:
Article