Crystallization and preliminary X-ray diffraction analysis of mouse prostaglandin F2α synthase, AKR1B3.
Acta Crystallogr Sect F Struct Biol Cryst Commun
; 67(Pt 12): 1630-2, 2011 Dec 01.
Article
em En
| MEDLINE
| ID: mdl-22139184
ABSTRACT
Aldo-keto reductase 1B3 (AKR1B3) catalyzes the NADPH-dependent reduction of prostaglandin H(2) (PGH(2)), which is a common intermediate of various prostanoids, to form PGF(2α). AKR1B3 also reduces PGH(2) to PGD(2) in the absence of NADPH. AKR1B3 produced in Escherichia coli was crystallized in complex with NADPH by the sitting-drop vapour-diffusion method. The crystal was tetragonal, belonging to space group P4(1)2(1)2 or P4(3)2(1)2, with unit-cell parameters a = b = 107.62, c = 120.76 Å. X-ray diffraction data were collected to 2.4 Å resolution at 100 K using a synchrotron-radiation source.
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1
Base de dados:
MEDLINE
Assunto principal:
Hidroxiprostaglandina Desidrogenases
Limite:
Animals
Idioma:
En
Ano de publicação:
2011
Tipo de documento:
Article