Histomorphometry of the ligaments using a generic-purpose image processing software, a new strategy for semi-automatized measurements.
J Digit Imaging
; 25(4): 527-36, 2012 Aug.
Article
em En
| MEDLINE
| ID: mdl-22143412
ABSTRACT
Gold chloride technique can be combined with Adobe Photoshop® software to yield a quantitative assessment of the different areas in heterogeneous structures as are ligament. A semi-automatized method based on the sum of two- and three-dimensional morphological criteria upon colorimetric criteria allows the identification and measurement of the area occupied by a structure of interest. It also allows the quantification of color intensity to differentiate structures with similar staining avidity, like vessels and nerves. This computer-assisted, semiquantitative procedure for computerized morphometry is relatively simple to perform. The accuracy, efficiency, and reproducibility of this method based on a commercially available imaging program were considered adequate when tested on the anterior cruciate ligament of the cat. Image normalization by trained observers using a commercially available software package designed for photography, applied to a sample randomly chosen, has provided the means of making reproducible measurements of heterogeneous structures.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Coloração e Rotulagem
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Processamento de Imagem Assistida por Computador
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Software
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Fotografação
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Técnicas Histológicas
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
En
Ano de publicação:
2012
Tipo de documento:
Article