PolyA RT-PCR-based quantification of microRNA by using universal TaqMan probe.
Biotechnol Lett
; 34(4): 627-33, 2012 Apr.
Article
em En
| MEDLINE
| ID: mdl-22160364
ABSTRACT
Quantification of microRNAs (miRNAs) in tissues under normal and pathological conditions is important for elucidating miRNA functions. Based on a PolyA RT-PCR method we have described (J Zhang et al. Biochem Biophys Res Commun 2008 377136-140), a modified miRNA quantification method was developed and validated using a universal TaqMan probe complementary to the reverse transcript primer. This method effectively detects miRNA expression in cell lines and tissues. The TaqMan probe is more accurate and reliable than the SYBR Green method since it was free from primer dimers. A series of miRNAs were tested in five different mouse tissues the method differentiated different miRNAs of the same family. This universal TaqMan probe-based PolyA RT-PCR method showed its advantages in precision, simplicity and high-throughput capability compared with other miRNA-detecting methods.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Poli A
/
Sondas de Oligonucleotídeos
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Reação em Cadeia da Polimerase
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MicroRNAs
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Biologia Molecular
Tipo de estudo:
Evaluation_studies
Limite:
Animals
Idioma:
En
Ano de publicação:
2012
Tipo de documento:
Article