N-terminal constraint activates the catalytic subunit of the DNA-dependent protein kinase in the absence of DNA or Ku.
Nucleic Acids Res
; 40(7): 2964-73, 2012 Apr.
Article
em En
| MEDLINE
| ID: mdl-22167471
ABSTRACT
The DNA-dependent protein kinase (DNA-PK) was identified as an activity and as its three component polypeptides 25 and 15 years ago, respectively. It has been exhaustively characterized as being absolutely dependent on free double stranded DNA ends (to which it is directed by its regulatory subunit, Ku) for its activation as a robust nuclear serine/threonine protein kinase. Here, we report the unexpected finding of robust DNA-PKcs activation by N-terminal constraint, independent of either DNA or its regulatory subunit Ku. These data suggest that an N-terminal conformational change (likely induced by DNA binding) induces enzymatic activation.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
DNA
/
Proteína Quinase Ativada por DNA
Tipo de estudo:
Prognostic_studies
Limite:
Animals
/
Humans
Idioma:
En
Ano de publicação:
2012
Tipo de documento:
Article