PCR identification of bacteria in blood culture does not fit the daily workflow of a routine microbiology laboratory.
J Clin Microbiol
; 50(3): 1031-3, 2012 Mar.
Article
em En
| MEDLINE
| ID: mdl-22170900
ABSTRACT
We have evaluated the GenoType blood culture assay (Hain Lifescience, Nehren, Germany) for the identification of bacteria in 233 positive blood cultures and assessed its suitability in the workflow of a routine microbiology laboratory. In 68/233 (29.2%) samples, the culture result could not be confirmed by the GenoType assay due to a lack of primers in the test, multiple organisms in the sample, or inconsistency with respect to the identification by culture. Although the GenoType blood culture assay gives satisfactory results for bacteria for which primers are available, there are difficulties in applying the test in the routine microbiology laboratory.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Bactérias
/
Sangue
/
Reação em Cadeia da Polimerase
/
Técnicas Bacteriológicas
/
Bacteriemia
/
Técnicas de Diagnóstico Molecular
/
Fluxo de Trabalho
Tipo de estudo:
Diagnostic_studies
/
Evaluation_studies
/
Prognostic_studies
Limite:
Humans
País como assunto:
Europa
Idioma:
En
Ano de publicação:
2012
Tipo de documento:
Article