Novel multiplexed HIV/simian immunodeficiency virus antibody detection assay.
Emerg Infect Dis
; 17(12): 2277-86, 2011 Dec.
Article
em En
| MEDLINE
| ID: mdl-22172157
ABSTRACT
Like most emerging infectious disease viruses, HIV is also of zoonotic origin. To assess the risk for cross-species transmission of simian immunodeficiency viruses (SIVs) from nonhuman primates to humans in the Democratic Republic of Congo, we collected 330 samples derived from nonhuman primate bushmeat at 3 remote forest sites. SIV prevalences were estimated by using a novel high-throughput assay that included 34 HIV and SIV antigens in a single well. Overall, 19% of nonhuman primate bushmeat was infected with SIVs, and new SIV lineages were identified. Highest SIV prevalences were seen in red-tailed guenons (25%) and Tshuapa red colobus monkeys (24%), representing the most common hunted primate species, thus increasing the likelihood for cross-species transmission. Additional studies are needed to determine whether other SIVs crossed the species barrier. With the newly developed assay, large-scale screening against many antigens is now easier and faster.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Imunoensaio
/
Anticorpos Anti-HIV
/
Vírus da Imunodeficiência Símia
/
Anticorpos Antivirais
Tipo de estudo:
Diagnostic_studies
País como assunto:
Africa
Idioma:
En
Ano de publicação:
2011
Tipo de documento:
Article