Fast mapping of biomolecular interfaces by Random Spin Labeling (RSL).
J Biomol Struct Dyn
; 29(4): 793-8, 2012.
Article
em En
| MEDLINE
| ID: mdl-22208279
ABSTRACT
Random spin labeling (RSL) is a method for rapid mapping of biomolecular interaction surfaces using an interaction partner with SL and an interaction partner enriched in (13)C or (15)N nuclei for paramagnetic relaxation enhanced NMR-based detection. The SL reaction is conducted in a manner resulting in a heterogeneous reaction product consisting of different populations of the protein carrying a varying number of spin labels at different positions. Preparation of the paramagnetic probe is complete within a few hours and hence much faster than site selective SL. RSL is applicable to tightly interacting systems but shows its particular strength when applied to systems involving weak or transient contacts.
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Base de dados:
MEDLINE
Assunto principal:
Marcadores de Spin
/
Espectroscopia de Ressonância Magnética
Tipo de estudo:
Clinical_trials
Idioma:
En
Ano de publicação:
2012
Tipo de documento:
Article