Developmental validation of RSID™-Semen: a lateral flow immunochromatographic strip test for the forensic detection of human semen.
J Forensic Sci
; 57(2): 489-99, 2012 Mar.
Article
em En
| MEDLINE
| ID: mdl-22211796
ABSTRACT
Tests for the identification of semen commonly involve the microscopic visualization of spermatozoa or assays for the presence of seminal markers such as acid phosphatase (AP) or prostate-specific antigen (PSA). Here, we describe the rapid stain identification kit for the identification of semen (RSID™-Semen), a lateral flow immunochromatographic strip test that uses two antihuman semenogelin monoclonal antibodies to detect the presence of semenogelin. The RSID™-Semen strip is specific for human semen, detecting <2.5 nL of semen, and does not cross-react with other human or nonhuman tissues tested. RSID™-Semen is more sensitive with certain forensic evidence samples containing mixtures of vaginal secretions and semen than either of the commercially available PSA-based forensic semen detection tests or tests that measure AP activity that were tested in parallel. The RSID™-Semen kit also allows sampling a fraction of a questioned stain while retaining the majority of the sample for further processing through short tandem repeat analysis.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Fitas Reagentes
/
Sêmen
/
Cromatografia de Afinidade
Tipo de estudo:
Diagnostic_studies
/
Prognostic_studies
Limite:
Animals
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Humans
/
Male
Idioma:
En
Ano de publicação:
2012
Tipo de documento:
Article