Controlled release of drugs from gradient hydrogels for high-throughput analysis of cell-drug interactions.
Anal Chem
; 84(3): 1302-9, 2012 Feb 07.
Article
em En
| MEDLINE
| ID: mdl-22220576
ABSTRACT
In this paper, we report a method to fabricate microengineered hydrogels that contain a concentration gradient of a drug for high-throughput analysis of cell-drug interactions. A microfluidic gradient generator was used to create a concentration gradient of okadaic acid (OA) as a model drug within poly(ethylene glycol) diacrylate hydrogels. These hydrogels were then incubated with MC3T3-E1 cell seeded glass slides to investigate the cell viability through the spatially controlled release of OA. The drug was released from the hydrogel in a gradient manner and induced a gradient of the cell viability. The drug concentration gradient containing hydrogels developed in this study have the potential to be used for drug discovery and diagnostics applications due to their ability to simultaneously test the effects of different concentrations of various chemicals.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Ácido Okadáico
/
Hidrogéis
/
Ensaios de Triagem em Larga Escala
Limite:
Animals
Idioma:
En
Ano de publicação:
2012
Tipo de documento:
Article