Mixed Giardia duodenalis assemblage infections in children and adults in South India.
Epidemiol Infect
; 140(11): 2023-7, 2012 Nov.
Article
em En
| MEDLINE
| ID: mdl-22225911
ABSTRACT
The assemblages of Giardia duodenalis in 25 children with and 25 children without diarrhoea and 24 adults with gastrointestinal symptoms in South India were determined. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) targeting the glutamate dehydrogenase (gdh), ß-giardin and triosephosphate isomerase (tpi) genes was used. The tpi PCR was the most sensitive and detected G. duodenalis in all 74 microscopy-positive samples, while gdh and ß-giardin PCR were positive in 62·2% and 56·8% of the samples. Assemblage B was predominant in both children and adults (82·4%) followed by assemblage AII (9·4%); assemblage AI was not detected. Infections with both assemblages A and B (detected by tpi PCR-RFLP) were seen exclusively in children and the mixed assemblage BIII and BIV (detected by gdh PCR-RFLP) was more common in children than adults (P=0·058).
Buscar no Google
Base de dados:
MEDLINE
Assunto principal:
Triose-Fosfato Isomerase
/
Polimorfismo de Fragmento de Restrição
/
Proteínas de Protozoários
/
DNA de Protozoário
/
Giardíase
/
Giardia lamblia
/
Glutamato Desidrogenase
Limite:
Adult
/
Child
/
Humans
País como assunto:
Asia
Idioma:
En
Ano de publicação:
2012
Tipo de documento:
Article