Coordinated regulation of IL-4 and IL-13 expression in human T cells: 3C analysis for DNA looping.
Biochem Biophys Res Commun
; 417(3): 996-1001, 2012 Jan 20.
Article
em En
| MEDLINE
| ID: mdl-22226971
ABSTRACT
Asthma is a chronic allergic disorder characterised by chronic inflammation. The balance of type I and type II (CD4+) T helper cells is of critical importance. In asthma there is an overexpression of T(H)2 cytokines, such as IL-4, IL-5 and IL-13. The genes encoding these cytokines are located together the same chromosomal region, 5q31.1 in humans. Here we confirm a central role for the transcription factors NFAT and GATA3 in the regulation of human IL-4 and IL-13. Chromatin Conformation Capture (3C) demonstrated the formation of specific ligation products containing spliced IL-4 and IL-13 DNA sequences in human T(H)2 polarised HuT-78 cells. This suggests that co-ordinate expression of T(H)2 cytokines, under the control of GATA3 and NFAT1 is due to the formation of a chromatin hub by DNA looping.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Asma
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Cromatina
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Regulação da Expressão Gênica
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Interleucina-4
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Células Th2
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Interleucina-13
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Fator de Transcrição GATA3
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Fatores de Transcrição NFATC
Limite:
Humans
Idioma:
En
Ano de publicação:
2012
Tipo de documento:
Article