Novel application of Ag nanoclusters in fluorescent imaging of human serum proteins after native polyacrylamide gel electrophoresis (PAGE).
Chemistry
; 18(5): 1432-7, 2012 Jan 27.
Article
em En
| MEDLINE
| ID: mdl-22249908
ABSTRACT
We have developed a novel application for DNA oligonucleotide-stabilized Ag nanoclusters in fluorescent imaging of human serum proteins after native polyacrylamide gel electrophoresis (PAGE). Oligonucleotide-stabilized Ag nanoclusters were used as fluorescent probes for direct detection of proteins after native PAGE. Some relatively low-abundance proteins, such as α-1-antichymotrypsin (ACT) and α-2-glycoprotein 1, zinc (ZAG) were easily detected by oligonucleotide-stabilized Ag nanocluster-based fluorescent imaging and identified by MS and MS/MS techniques, without the need of expensive antibodies or tedious immunoassay procedures. The pH condition for the oligonucleotide-stabilized Ag nanocluster solution was optimized and the possible mechanism of interaction between proteins and DNA oligonucleotide-stabilized Ag nanoclusters was analyzed. As a novel fluorescent detection method it is simple, fast, nontoxic and sensitive, and it shows great analytical potential in proteome research and in biochemistry.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Proteínas Sanguíneas
/
Nanoestruturas
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Eletroforese em Gel de Poliacrilamida
/
Corantes Fluorescentes
Limite:
Humans
Idioma:
En
Ano de publicação:
2012
Tipo de documento:
Article