The application of amine-terminated silicon quantum dots on the imaging of human serum proteins after polyacrylamide gel electrophoresis (PAGE).
Chemistry
; 18(5): 1438-43, 2012 Jan 27.
Article
em En
| MEDLINE
| ID: mdl-22249969
ABSTRACT
Novel amine-terminated silicon (Si) quantum dots (QDs) were synthesized and applied for the detection of human serum proteins on gels directly after polyacrylamide gel electrophoresis (PAGE). The diameter of these stable amine-terminated Siâ
QDs was in the range of 0.5-2.0 nm. In this study, the fluorescent imaging conditions, such as the buffer solution, pH value, buffer concentration and quantity of Siâ
QDs, were optimized and the possible mechanisms of Siâ
QDs-protein interaction were analyzed. The mode of Siâ
QDs and human serum albumin association was found to occur by hydrogen bond interactions; this was probably attributed to the interaction between the amino group of amine-terminated Siâ
QDs and the carboxyl group of proteins. Meanwhile, human serum proteins separated by native 1D and native 2D electrophoresis were detected by Si QD-based fluorescent imaging. Some proteins, such as isoform 1 of α-1-antitrypsin, complement C3 (Fragment) and hemopexin, which were identified by mass spectrometry (MS), were easily detected by using Siâ
QDs, but not with CBB-R250 staining. The Siâ
QDs-based fluorescent imaging technique with high resolution is a sensitive and dependable method for direct detection of human serum proteins, and has enormous potential in clinical diagnosis.
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Base de dados:
MEDLINE
Assunto principal:
Silício
/
Proteínas Sanguíneas
/
Pontos Quânticos
/
Eletroforese em Gel de Poliacrilamida
/
Aminas
Limite:
Humans
Idioma:
En
Ano de publicação:
2012
Tipo de documento:
Article