Micro-sized syringes for single-cell fluidic access integrated on a micro-electrode array CMOS chip.
Annu Int Conf IEEE Eng Med Biol Soc
; 2011: 7650-3, 2011.
Article
em En
| MEDLINE
| ID: mdl-22256110
ABSTRACT
Very-large scale integration and micro-machining have enabled the development of novel platforms for advanced and automated examination of cells and tissues in vitro. In this paper, we present a CMOS chip designed in a commercial 0.18 µm technology with integrated micro-syringes combined with micro-nail shaped electrodes and readout electronics. The micro-syringes could be individually addressed by a through-wafer micro-fluidic channel with an inner diameter of 1 µm. We demonstrated the functionality of the micro-fluidic access by diffusion of fluorescent species through the channels. Further, hippocampal neurons were cultured on top of an array of micro-syringes, and focused ion beam-scanning electron microscopy cross-sections revealed protrusion of the cells inside the channels, creating a strong interface between the membrane and the chip surface. This principle demonstrates a first step towards a novel type of automated in vitro platforms, allowing local delivery of substances to cells or advanced planar patch clamping.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Óxidos
/
Semicondutores
/
Seringas
/
Técnicas Analíticas Microfluídicas
/
Análise de Célula Única
/
Miniaturização
Limite:
Animals
Idioma:
En
Ano de publicação:
2011
Tipo de documento:
Article