OMIP-006: phenotypic subset analysis of human T regulatory cells via polychromatic flow cytometry.
Cytometry A
; 81(4): 281-3, 2012 Apr.
Article
em En
| MEDLINE
| ID: mdl-22319016
ABSTRACT
This panel was optimized for the enumeration and phenotypic characterization of T regulatory cells (Tregs) within the CD4⺠T-cell pool using human peripheral blood mononuclear cells (PBMC) using intranuclear and intracellular staining methods. The panel was optimized for HIV⺠clinical trial specimens through the use of HIV-infected and normal donor PBMC. Because the panel is to be used in the context of testing cryopreserved PBMC obtained from multiple sites participating in clinical trials, it was essential to develop an assay that performed well using cryopreserved PBMC. Other tissue types have not been tested.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Fenótipo
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Leucócitos Mononucleares
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Linfócitos T Reguladores
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Citometria de Fluxo
Limite:
Humans
Idioma:
En
Ano de publicação:
2012
Tipo de documento:
Article