1α,25-dihydroxyvitamin D3 promotes CD200 expression by human peripheral and airway-resident T cells.
Thorax
; 67(7): 574-81, 2012 Jul.
Article
em En
| MEDLINE
| ID: mdl-22334534
ABSTRACT
BACKGROUND:
CD200, a cell-surface immunoglobulin-like molecule expressed by immune and stromal cells, dampens the pro-inflammatory activity of tissue-resident innate cells via its receptor, CD200R. This interaction appears critical for peripheral immune tolerance, particularly in the airways where excessive inflammation is undesirable. Vitamin D contributes to pulmonary health and promotes regulatory immune pathways, therefore its influence on CD200 and CD200R was investigated.METHODS:
CD200 and CD200R expression were assessed by qPCR and immunoreactivity of human lymphoid, myeloid and epithelial cells following 1α,25-dihydroxyvitamin D3 (1α,25VitD3) exposure in vitro and in peripheral T cells following 1α,25VitD3 oral ingestion in vivo. The effect of 1α25VitD3 was also assessed in human airway-resident cells.RESULTS:
1α25VitD3 potently upregulated CD200 on peripheral human CD4+ T cells in vitro, and in vivo there was a trend towards upregulation in healthy, but not asthmatic individuals. CD200R expression was not modulated in any cells studied. CD200 induction was observed to a lesser extent in CD8+ T cells and not in B cells or airway epithelium. T cells isolated from the human airway also responded strongly to 1α25VitD3 to upregulate CD200.CONCLUSIONS:
The capacity of 1α,25-dihydroxyvitamin D3 to induce CD200 expression by peripheral and respiratory tract T cells identifies an additional pathway via which vitamin D can restrain inflammation in the airways to maintain respiratory health.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Vitamina D
/
RNA Mensageiro
/
Linfócitos T
/
Antígenos CD
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Regulação para Cima
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Mucosa Respiratória
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Tolerância Imunológica
Limite:
Child
/
Humans
Idioma:
En
Ano de publicação:
2012
Tipo de documento:
Article