[Regulatory role of Syk gene on vascular endothelial growth factor C expression in breast cancer].
Zhonghua Bing Li Xue Za Zhi
; 40(12): 805-9, 2011 Dec.
Article
em Zh
| MEDLINE
| ID: mdl-22336204
ABSTRACT
OBJECTIVE:
To investigate the effect of Syk on the VEGF-C expression in breast cancer.METHODS:
Immunohistochemical EnVision method was used to detect the protein expression of Syk, NFκB and VEGF-C in breast carcinoma; and the relationship between protein expression of Syk, NFκB, VEGF-C and lymph node metastasis was analysed. MDA-MB-231 cells were transfected with pcDNA3.1(-)-Syk, and the effect of Syk gene on the VEGF-C and NFκB expression was determined.RESULTS:
In the lymph node metastatic group, a lower expression rate of Syk and higher expression rate of VEGF-C and NFκB were detected as compared to the non-metastatic group. The expression of Syk was negatively associated with NFκB (r = -0.448, P = 0.002) and VEGF-C (r = -0.620, P = 0.000) expression, and VEGF-C was associated with the nuclear expression of NFκB (r = 0.310, P = 0.036). Compared with the non-transfected cells, the pcDNA3.1(-)-Syk transfected MDA-MB-231 cells showed significantly lower transcriptional level of VEGF-C mRNA, expression level of VEGF-C protein and NFκB activity (P < 0.05).CONCLUSIONS:
Syk may play an important role in the lymph node metastasis of breast cancer. It may down-regulate the expression of VEGF-C by inhibiting the activity of NFκB, which thus suppresses lymph node metastasis of breast cancer.
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Base de dados:
MEDLINE
Assunto principal:
Proteínas Tirosina Quinases
/
Neoplasias da Mama
/
Carcinoma Ductal de Mama
/
Fator C de Crescimento do Endotélio Vascular
/
Peptídeos e Proteínas de Sinalização Intracelular
Limite:
Adult
/
Aged
/
Female
/
Humans
/
Middle aged
Idioma:
Zh
Ano de publicação:
2011
Tipo de documento:
Article