Protein-protein interactions as a proxy to monitor conformational changes and activation states of the tomato resistance protein I-2.
J Exp Bot
; 63(8): 3047-60, 2012 May.
Article
em En
| MEDLINE
| ID: mdl-22345637
ABSTRACT
Plant resistance proteins (R) are involved in pathogen recognition and subsequent initiation of defence responses. Their activity is regulated by inter- and intramolecular interactions. In a yeast two-hybrid screen two clones (I2I-1 and I2I-2) specifically interacting with I-2, a Fusarium oxysporum f. sp. lycopersici resistance protein of the CC-NB-LRR family, were identified. Sequence analysis revealed that I2I-1 belongs to the Formin gene family (SlFormin) whereas I2I-2 has homology to translin-associated protein X (SlTrax). SlFormin required only the N-terminal CC I-2 domain for binding, whereas SlTrax required both I-2 CC and part of the NB-ARC domain. Tomato plants stably silenced for these interactors were not compromised in I-2-mediated disease resistance. When extended or mutated forms of I-2 were used as baits, distinct and often opposite, interaction patterns with the two interactors were observed. These interaction patterns correlated with the proposed activation state of I-2 implying that active and inactive R proteins adopt distinct conformations. It is concluded that the yeast two hybrid system can be used as a proxy to monitor these different conformational states.
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Base de dados:
MEDLINE
Assunto principal:
Doenças das Plantas
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Proteínas de Plantas
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Solanum lycopersicum
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Resistência à Doença
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Mapas de Interação de Proteínas
Idioma:
En
Ano de publicação:
2012
Tipo de documento:
Article