Isolation of ubiquitylated proteins using tandem ubiquitin-binding entities.
Methods Mol Biol
; 832: 173-83, 2012.
Article
em En
| MEDLINE
| ID: mdl-22350885
ABSTRACT
Studying postubiquitylation events has always been a difficult task due to the labile nature of these posttranslational modifications. When utilized in tandem, ubiquitin-binding entities (TUBEs) not only increase up to thousand times the affinity for poly-ubiquitin chains but also protect ubiquitylated proteins from the action of the proteasome and de-ubiquitylating enzymes.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Cromatografia de Afinidade
/
Poliubiquitina
Idioma:
En
Ano de publicação:
2012
Tipo de documento:
Article