[Study of proteomic profile of Danio rerio embryos using one-dimensional electrophoresis and mass spectrometry].
Biomed Khim
; 57(6): 593-603, 2011.
Article
em Ru
| MEDLINE
| ID: mdl-22359915
ABSTRACT
In the present study, a proteomic technology combining one-dimensional gel electrophoresis (1DE) with subsequent mass spectrometry (MALDI-TOF-PMF) has been successfully applied for revelation of changes in the protein profile of zebrafish (Danio rerio) 52 hpf embryos. Prior to 1DE separation of zebrafish embryonic proteins, the procedure for obtaining embryos homogenate was optimized by ultrasonic treatment. A total of 84 proteins, including 15 vitellogenins, were identified. It was shown that growing ofzebrafish embryos in the medium with doxorubicin (DOX) stimulated Caspase-3 induction and promoted the disappearance of cardiac troponins, both these findings being consistent with literature data on doxorubicin-induced cardiotoxicity. The 1DE-based proteomic mapping approach proposed herein enabled not only to identify proteins but also to register those changes in embryos' proteomic profile that were caused by doxorubicin.
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Base de dados:
MEDLINE
Assunto principal:
Peixe-Zebra
/
Proteoma
/
Proteínas de Peixe-Zebra
/
Embrião não Mamífero
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
Ru
Ano de publicação:
2011
Tipo de documento:
Article