Circular DNA genomics (circomics) exemplified for geminiviruses in bean crops and weeds of northeastern Brazil.
Virology
; 427(2): 151-7, 2012 Jun 05.
Article
em En
| MEDLINE
| ID: mdl-22397740
ABSTRACT
Circomics was coined to describe the combination of rolling circle amplification (RCA), restriction fragment length polymorphism (RFLP) and pyro-sequencing to investigate the genome structures of small circular DNAs. A batch procedure is described using 61 plant samples from Asia, South America and Central America which revealed 83 contig sequences of geminiviral DNA components and 4 contig sequences of DNA satellites. The usefulness of this approach is validated for the Brazilian begomoviruses, and the sequence fidelity is determined by comparing the results with those of conventional cloning and sequencing of Bolivian begomoviruses reported recently. Therefore, circomics has been proven to be a major step forward to economize costs and labor and to characterize reliably geminiviral genomes in their population structure of the quasispecies.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
DNA Circular
/
DNA Viral
/
Geminiviridae
/
Técnicas de Amplificação de Ácido Nucleico
/
Plantas Daninhas
/
Fabaceae
País como assunto:
America do sul
/
Brasil
Idioma:
En
Ano de publicação:
2012
Tipo de documento:
Article