Optimization and validation of organochlorine compounds in adipose tissue by SPE-gas chromatography.
Biomed Chromatogr
; 26(12): 1494-501, 2012 Dec.
Article
em En
| MEDLINE
| ID: mdl-22407478
ABSTRACT
Scientific evidence has shown an association between organochlorine compounds (OCC) exposure and human health hazards. Concerning this, OCC detection in human adipose samples has to be considered a public health priority. This study evaluated the efficacy of various solid-phase extraction (SPE) and cleanup methods for OCC determination in human adipose tissue. Octadecylsilyl endcapped (C18-E), benzenesulfonic acid modified silica cation exchanger (SA), poly(styrene-divinylbenzene (EN) and EN/RP18 SPE sorbents were evaluated. The relative sample cleanup provided by these SPE columns was evaluated using gas chromatography with electron capture detection (GC-ECD). The C18-E columns with strong homogenization were found to provide the most effective cleanup, removing the greatest amount of interfering substance, and simultaneously ensuring good analyte recoveries higher than 70%. Recoveries > 70% with standard deviations (SD) < 15% were obtained for all compounds under the selected conditions. Method detection limits were in the 0.003-0.009 mg/kg range. The positive samples were confirmed by gas chromatography coupled with tandem mass spectrometry (GC-MS/MS). The highest percentage found of the OCC in real samples corresponded to HCB, o,p'-DDT and methoxychlor, which were detected in 80 and 95% of samples analyzed respectively.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Resíduos de Praguicidas
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Tecido Adiposo
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Cromatografia Gasosa
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Extração em Fase Sólida
/
Hidrocarbonetos Clorados
Limite:
Humans
Idioma:
En
Ano de publicação:
2012
Tipo de documento:
Article