Functional expression of an orchid fragrance gene in Lactococcus lactis.
Int J Mol Sci
; 13(2): 1582-1597, 2012.
Article
em En
| MEDLINE
| ID: mdl-22408409
ABSTRACT
Vanda Mimi Palmer (VMP), an orchid hybrid of Vanda tesselata and Vanda Tan Chay Yan is a highly scented tropical orchid which blooms all year round. Previous studies revealed that VMP produces a variety of isoprenoid volatiles during daylight. Isoprenoids are well known to contribute significantly to the scent of most fragrant plants. They are a large group of secondary metabolites which may possess valuable characteristics such as flavor, fragrance and toxicity and are produced via two pathways, the mevalonate (MVA) pathway or/and the 2-C-methyl-D-erythritol-4-phosphate (MEP) pathway. In this study, a sesquiterpene synthase gene denoted VMPSTS, previously isolated from a floral cDNA library of VMP was cloned and expressed in Lactococcus lactis to characterize the functionality of the protein. L. lactis, a food grade bacterium which utilizes the mevalonate pathway for isoprenoid production was found to be a suitable host for the characterization of plant terpene synthases. Through recombinant expression of VMPSTS, it was revealed that VMPSTS produced multiple sesquiterpenes and germacrene D dominates its profile.
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Base de dados:
MEDLINE
Assunto principal:
Proteínas de Plantas
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Óleos Voláteis
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Lactococcus lactis
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Genes de Plantas
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Magnoliopsida
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Alquil e Aril Transferases
Idioma:
En
Ano de publicação:
2012
Tipo de documento:
Article