Tryparedoxin peroxidases and superoxide dismutases expression as well as ROS release are related to Trypanosoma cruzi epimastigotes growth phases.
Arch Biochem Biophys
; 520(2): 117-22, 2012 Apr 15.
Article
em En
| MEDLINE
| ID: mdl-22425758
ABSTRACT
Trypanosoma cruzi's antioxidant system is unique and relevant to the parasite. In this study, quantitative assays were performed to determine cytosolic and mitochondrial tryparedoxin peroxidases and superoxide dismutases expression (TcCPx, TcMPx, SODB and SODA) in correlation to H(2)O(2) release and O(2)(-) production. Differences were observed regarding H(2)O(2) release and O(2)(-) production between strains and along the growth curve. All of the enzymes studied exhibited varied expression as a function of time in culture. Although at lower levels, the Y strain exhibited the same pattern of Tulahuen 2 enzyme expression for all of the proteins studied, except SODA. In the stationary phase, the degree of expression of all of the enzymes in the Y strain returned to similar levels as those detected in the log phase with the exception of TcCPx and SODA. In Tulahuen 2, a higher expression of TcMPx, SODA and SODB was detected in the early stationary phase, and a slight decrease was observed in the late stationary phase for each enzyme, excluding TcMPx, which exhibited a marked decrease, and TcCPx, which increased its level. Because of the significance of ROS in redox signaling, these differences in enzyme expression underscore the importance of these parameters for epimastigote proliferation.
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Base de dados:
MEDLINE
Assunto principal:
Peroxidases
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Superóxido Dismutase
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Trypanosoma cruzi
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Regulação Enzimológica da Expressão Gênica
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Proteínas de Protozoários
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Espécies Reativas de Oxigênio
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Regulação da Expressão Gênica no Desenvolvimento
Idioma:
En
Ano de publicação:
2012
Tipo de documento:
Article