Fluorescence-based active site probes for profiling deubiquitinating enzymes.
Org Biomol Chem
; 10(17): 3379-83, 2012 May 07.
Article
em En
| MEDLINE
| ID: mdl-22453277
ABSTRACT
Novel ubiquitin-based active site probes including a fluorescent tag have been developed and evaluated. A new, functionalizable electrophilic trap is utilized allowing for late stage diversification of the probe. Attachment of fluorescent dyes allowed direct detection of endogenous deubiquitinating enzyme (DUB) activities in cell extracts by in-gel fluorescence imaging.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Endopeptidases
/
Domínio Catalítico
/
Ensaios Enzimáticos
/
Corantes Fluorescentes
Tipo de estudo:
Prognostic_studies
Limite:
Humans
Idioma:
En
Ano de publicação:
2012
Tipo de documento:
Article