Preparation and determination of optical purity of γ-lysine modified peptide nucleic acid analogues.
Arch Pharm Res
; 35(3): 517-22, 2012 Mar.
Article
em En
| MEDLINE
| ID: mdl-22477199
ABSTRACT
Peptide nucleic acids (PNAs) are DNA analogues in which the nucleic acid backbone is replaced by a pseudopeptide backbone and nucleobases are attached to the backbone by methylene carbonyl linkers. γ-Carbon modification of the PNA structure allows monomers, and subsequently oligomers, with improved properties to be obtained. In this study, we report the convenient synthesis of γ-lysine-modified PNA monomers for pyrimidine bases (thymine and cytosine) with high optical purity (> 99.5%) and direct enantiomer separation of γ-lysine-modified PNA analogs, using chiral HPLC to determine the optical purity.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Ácidos Nucleicos Peptídicos
/
Lisina
Idioma:
En
Ano de publicação:
2012
Tipo de documento:
Article