Preparative 2D Gel Electrophoresis with Immobilized pH Gradients: Rehydration of IPG Strips for Isoelectric Focusing of Proteins.
CSH Protoc
; 2006(5)2006 Oct 01.
Article
em En
| MEDLINE
| ID: mdl-22485958
ABSTRACT
INTRODUCTIONThis protocol describes a method for rehydration of IPG gel strips in preparation for their use for isoelectric focusing (IEF) on immobilized pH gradient (IPG) gels. Following rehydration, IEF can be performed using either a flatbed unit or a self-contained instrument.
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MEDLINE
Idioma:
En
Ano de publicação:
2006
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Article