Stabilization of collagen tissues by photocrosslinking.
J Biomed Mater Res A
; 100(9): 2239-43, 2012 Sep.
Article
em En
| MEDLINE
| ID: mdl-22492704
ABSTRACT
Photocrosslinking, using 2 mM Ru(II)(bpy)(3)Cl(2) and various concentrations of sodium persulfate with irradiation by blue light, â¼455 nm, has been shown to be a rapid and effective method for crosslinking various tissues tendon, amnion membrane, pericardium, and heart valve leaflet. The presence of new crosslinking was demonstrated by the increase in the shrinkage temperature of these tissues. In all the cases, increase in the shrinkage temperatures were seen, although at higher sodium persulfate concentrations, for example, 100 mM, both with and without the Ru(II)(bpy)(3)Cl(2) catalyst, some degradation of the collagenous tissues was found. The effectiveness of this photocrosslinking method when used with tissues was also shown through the increase in the break strength of tissues after crosslinking, and by the reduction of protein that could be extracted by urea. In solution studies, dityrosine has been shown to be formed during photocrosslinking. With tissues, Western blotting showed the presence of new dityrosine crosslinked proteins.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Compostos Organometálicos
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Materiais Biocompatíveis
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Colágeno
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Reagentes de Ligações Cruzadas
Limite:
Animals
Idioma:
En
Ano de publicação:
2012
Tipo de documento:
Article