Standardization and quality controls for the methylated DNA immunoprecipitation technique.
Epigenetics
; 7(6): 615-25, 2012 Jun 01.
Article
em En
| MEDLINE
| ID: mdl-22507898
ABSTRACT
MeDIP (Methylated DNA Immunoprecipitation) is a relatively recent technique aimed to enrich the methylated fraction of DNA with an antibody directed against 5-methyl-cytosine. MeDIP processed samples are suitable for investigation of the methylation status of specific genomic loci and for performing genome-wide screening when hybridized to DNA methylation microarrays or analyzed by deep sequencing. Here, we describe a standardization protocol and quality controls to assess the specificity, reproducibility and efficiency of the MeDIP procedure. These may have utility when comparing results between samples and experiments within laboratories and between laboratories.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Metilação de DNA
/
Análise de Sequência com Séries de Oligonucleotídeos
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Imunoprecipitação
Idioma:
En
Ano de publicação:
2012
Tipo de documento:
Article