Interactions of PRP2 protein with pre-mRNA splicing complexes in Saccharomyces cerevisiae.
Nucleic Acids Res
; 18(22): 6559-64, 1990 Nov 25.
Article
em En
| MEDLINE
| ID: mdl-2251118
ABSTRACT
PRP2 protein of Saccharomyces cerevisiae is required for the pre-mRNA splicing reaction but not for the early stages of spliceosome assembly. Using anti-PRP2 antibodies we demonstrate that PRP2 protein is associated with spliceosomes prior to, and throughout step 1 of the splicing reaction. Heat-inactivated prp2 protein, by contrast, does not seem to associate with spliceosomes. By elution of electrophoretically distinct spliceosomal complexes from non-denaturing gels we identify the specific complex with which PRP2 initially interacts in the pathway of spliceosome assembly.
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Base de dados:
MEDLINE
Assunto principal:
Saccharomyces cerevisiae
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Proteínas Fúngicas
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Precursores de RNA
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Splicing de RNA
Idioma:
En
Ano de publicação:
1990
Tipo de documento:
Article