Variability of mRNA abundance of leukemia inhibitory factor gene (LIF) in porcine ovary, oviduct and uterus tissues.

ABSTRACT

The leukemia inhibitory factor (LIF) gene encodes a pleiotropic cytokine which is produced by the endometrium and plays an important role in implantation and early embryonic development. Because of its function, LIF gene is considered as a candidate gene for litter size in many mammalian species including pig. The aim of present study was to evaluate the expression of LIF gene in the porcine ovary, oviduct and two regions of uterus (corpus uteri, cornu uteri) in prepubertal and pubertal gilts. In order to precise estimation of LIF transcript abundance we evaluated the stability of expression for several candidate housekeeping genes in investigated tissues across different breeds and different stage of oestrus cycle. The geNorm analysis indicated that the most stable reference genes across analyzed tissues were OAZ1 and RPL27. The analysis conducted separately for each tissue confirmed that the most stable gene was OAZ1 in all tissues expect oviduct (the most stable was RPL27 gene). In prepubertal pigs, the highest level of the LIF expression was obtained in both regions of uterus compare to ovary and oviduct tissues (P < 0.01). A similar trend in LIF expression pattern was observed in follicular phase-the significantly highest transcript level was obtained in cornu uteri, it was about ninefold higher than in ovary (P < 0.05). In luteal stage the highest expression was in corpus uteri. In pig, the high expression in luteal phases suggested that, LIF may be mainly secreted in respond to the increased of progesterone concentration and it can be connected with the preparation of the uterus for implantation.