Vitamin D receptor upregulation in alloreactive human T cells.
Hum Immunol
; 73(7): 693-8, 2012 Jul.
Article
em En
| MEDLINE
| ID: mdl-22548720
ABSTRACT
Vitamin D deficiency is adversely associated with diseases characterized by inflammation. The combination of the high incidence of vitamin D deficiency in patients undergoing allogeneic stem cell transplants (SCT) and the potential role of vitamin D deficiency in influencing graft-versus-host disease led us to further characterize the expression of VDR on alloreactive T cells. We hypothesized that vitamin D receptor expression may directly regulate alloreactive T cell responses. To overcome existing limitations in measuring VDR in bulk cellular populations, we developed a flow cytometric assay to measure cytoplasmic VDR in human T cells. Upon stimulation, VDR was expressed extremely early and exhibited sustained upregulation with chronic stimulation. VDR expression was also coupled to cytokine production, proliferation, and ERK1/2 phosphorylation. In addition, VDR exhibited a maturation stage-specific pattern of expression, with greatest expression on cells known to mediate GVHD, naïve and early memory T cells. Alloreactive T cells upregulated VDR, whereas the nonreactive T cells did not. Finally, repletion of vitamin D in vitro was sufficient to significantly reduce alloreactive T cell responses. These data suggest that vitamin D effects on T cells may be important in reducing graft versus host disease (GVHD) in the allogeneic stem cell transplant setting.
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Linfócitos T
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Subpopulações de Linfócitos T
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Receptores de Calcitriol
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Transplante de Células-Tronco
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Doença Enxerto-Hospedeiro
Limite:
Humans
Idioma:
En
Ano de publicação:
2012
Tipo de documento:
Article