High-strength silk protein scaffolds for bone repair.
Proc Natl Acad Sci U S A
; 109(20): 7699-704, 2012 May 15.
Article
em En
| MEDLINE
| ID: mdl-22552231
ABSTRACT
Biomaterials for bone tissue regeneration represent a major focus of orthopedic research. However, only a handful of polymeric biomaterials are utilized today because of their failure to address critical issues like compressive strength for load-bearing bone grafts. In this study development of a high compressive strength (~13 MPa hydrated state) polymeric bone composite materials is reported, based on silk protein-protein interfacial bonding. Micron-sized silk fibers (10-600 µm) obtained utilizing alkali hydrolysis were used as reinforcement in a compact fiber composite with tunable compressive strength, surface roughness, and porosity based on the fiber length included. A combination of surface roughness, porosity, and scaffold stiffness favored human bone marrow-derived mesenchymal stem cell differentiation toward bone-like tissue in vitro based on biochemical and gene expression for bone markers. Further, minimal in vivo immunomodulatory responses suggested compatibility of the fabricated silk-fiber-reinforced composite matrices for bone engineering applications.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Bombyx
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Materiais Biocompatíveis
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Regeneração Óssea
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Engenharia Tecidual
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Medicina Regenerativa
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Seda
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Alicerces Teciduais
Limite:
Animals
Idioma:
En
Ano de publicação:
2012
Tipo de documento:
Article